Quercetin and Kaempferol as Multi-Targeting Antidiabetic Agents against Mouse Model of Chemically Induced Type 2 Diabetes

Mrs. N DEEPA RAMANI, Mr. SHAIK RABBANI BASHA , Dr. SYED MOHAMMED

» doi: http://https://ijpsl.co.in//.2023.v13.i04.pp12-32

Abstract

Finding effective, safe medications that address several aspects of the metabolic condition known as diabetes is an urgent medical need. The potential of quercetin and kaempferol as multitargeting antidiabetic agents was examined in this research. Using pharmacokinetic and docking software (AutoDock Vina 1.1.2), the druggability and binding affinities towards several antidiabetic targets were investigated for both drugs. According to our research, quercetin and kaempferol have good ADMET (absorption, distribution, metabolism, excretion, and toxicity) profiles and follow Lipinski's rule of five. In comparison to metformin, the positive control, both compounds exhibited stronger binding affinities for C-reactive protein (CRP), interleukin-1 (IL-1), dipeptidyl peptidase-4 (DPP-IV), peroxisome proliferator-activated receptor gamma (PPARG), protein tyrosine phosphatase (PTP), and sodium-glucose co-transporter-1 (SGLT-1). Kaempferol reduced ?-amylase activity (in vitro) to a degree of 37.43 ± 0.42% and quercetin to a degree of 20.30 ± 0.49. In diabetic mice caused by streptozotocin-nicotinamide (STZ-NA), their oral supplementation led to a substantial decrease in blood glucose levels (p < 0.001), an improvement in lipid profile (p < 0.001), and an enhancement in total antioxidant status (p < 0.01). In addition, the growth of cancer cells Huh-7 and HepG2 was considerably reduced by both compounds (p < 0.0001), but the viability of the non-cancer Vero cell line was unaffected. Finally, compared to metformin, quercetin and kaempferol had stronger binding affinities to a variety of targets. Both compounds show promise for future research in diabetes treatment due to their antidiabetic capabilities in vitro and in vivo, as well as their anticancer activity. Maybe since they both target the same receptors, the two medications did not work together synergistically.